Abts Substrate. It is useful in elisa assay because of its high sensitivity, specificity, and reproducibility. The expected appearance of the solution is colorless to light green.
Peroxidase substrate general notes this product is manufactured by biovision, an abcam company and was previously called 1212 abts™ solution. Upon stopping, tmb changes in color from blue to yellow, while opd changes from yellow to orange. Acceptable background absorbance of unreacted substrate is ≤0.025, as measured at 405nm.
Good laboratory technique should be used when handling.
Abts peroxidase substrate (12 ml) $ 5.00. Peroxidase substrate general notes this product is manufactured by biovision, an abcam company and was previously called 1212 abts™ solution. Just prior to use, add 200 l abts solution and 10 l of 30% h 2 o 2 to 10 ml substrate buffer.
It is useful in elisa assay because of its high sensitivity, specificity, and reproducibility.
Sales on this site ship only to the us and canada. The addition of 100 µl (or an equal volume) of stop solution to the microwell plate will halt color development. The point at which the substrate reaction is stopped is often determined by the elisa reader.
Abts reacts with hydrogen peroxide and hrp to yield a green, soluble product.
Abts substrate remains green in color after addition of stop solution. Use absorbent materials if needed and dispose into a chemical waste container. Upon stopping, tmb changes in color from blue to yellow, while opd changes from yellow to orange.
Abts is not as sensitive as opd or tmb and it may take some time to properly develop its color.
Acceptable background absorbance of unreacted substrate is ≤0.025, as measured at 405nm. Readings are higher for tmb substrate th an for opd after stopping. Consequently, abts is not as often used as either opd or tmb.
Stopped reaction should be read within 30 minutes.
Abts is a highly water soluble and chemically stable compound. When to stop substrate reaction: Abts one component hrp microwell substrate offers sensitive detection of horseradish peroxidase in microwell applications.
Abts Substrate. It is useful in elisa assay because of its high sensitivity, specificity, and reproducibility. The expected appearance of the solution is colorless to light green.
Peroxidase substrate general notes this product is manufactured by biovision, an abcam company and was previously called 1212 abts™ solution. Upon stopping, tmb changes in color from blue to yellow, while opd changes from yellow to orange. Acceptable background absorbance of unreacted substrate is ≤0.025, as measured at 405nm.
Good laboratory technique should be used when handling.
Abts peroxidase substrate (12 ml) $ 5.00. Peroxidase substrate general notes this product is manufactured by biovision, an abcam company and was previously called 1212 abts™ solution. Just prior to use, add 200 l abts solution and 10 l of 30% h 2 o 2 to 10 ml substrate buffer.
It is useful in elisa assay because of its high sensitivity, specificity, and reproducibility.
Sales on this site ship only to the us and canada. The addition of 100 µl (or an equal volume) of stop solution to the microwell plate will halt color development. The point at which the substrate reaction is stopped is often determined by the elisa reader.
Abts reacts with hydrogen peroxide and hrp to yield a green, soluble product.
Abts substrate remains green in color after addition of stop solution. Use absorbent materials if needed and dispose into a chemical waste container. Upon stopping, tmb changes in color from blue to yellow, while opd changes from yellow to orange.
Abts is not as sensitive as opd or tmb and it may take some time to properly develop its color.
Acceptable background absorbance of unreacted substrate is ≤0.025, as measured at 405nm. Readings are higher for tmb substrate th an for opd after stopping. Consequently, abts is not as often used as either opd or tmb.
Stopped reaction should be read within 30 minutes.
Abts is a highly water soluble and chemically stable compound. When to stop substrate reaction: Abts one component hrp microwell substrate offers sensitive detection of horseradish peroxidase in microwell applications.
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